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PROGRAMA PRINCIPAL Jueves 19 de Octubre 19:00 Inscripción, registro y entrega de documentación 21:00 Recepción de bienvenida Viernes 20 de Octubre 9:30 Acto inaugural SESIÓN PLENARIA 1: TECNOLOGÍAS SEMINALES Moderador: Juan Carlos Domínguez Fernández-Tejerina 10:00 ¿Se puede incrementar el valor predictivo de la evaluación seminal? Heriberto Rodriguez-Martinez. Division of Comparative Reproduction, Obstetrics and Udder Health, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. 10:45 Gestión de los recursos energéticos y supervivencia en refrigeración de los espermatozoides mamíferos. Joan Enric Rodríguez Gil. Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain. 11:15 Tecnologías seminales en cría canina. FJ Peña, I Núñez-Martínez and JM Morán. Reproduction and Obstetrics Veterinary Teaching Hospital University of Extremadura, Cáceres, Spain. 12:00 Descanso/café 12:30 Comunicaciones orales I Moderador: Santos Argüello. Abstract OC1 Effects of cryopreservation on the expression of Glut-3, Glut-5 and As-A proteins in Iberian boar sperm membranes. S Sancho1,3, I Casas1, E Pinart1, M Briz1, N Garcia-Gil1, J Bassols1, A Pruneda1, E Bussalleu1, M Yeste1, E Flores2, JE Rodríguez-Gil2, H. Ekwall3, H. Rodríguez-Martínez3, S Bonet1 1 Biotechnology of Porcine Reproduction, Faculty of Sciences-INTEA, University of Girona, Spain. 2 Unit of Animal Reproduction, Dept. of Animal Medicine and Surgery, Autonomous University of Barcelona, Spain. 3 Division of Comparative Reproduction, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. Abstract OC2 Seasonality affects on sperm motility kinematic parameters of Murciano-Granadina bucks. D Abdelwahab, MM Rivera, JE Rodríguez-Gil, T Rigau. Dept. Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain. Abstract OC3 Morphometry characterisation of Catalan donkey spermatozoa and Identification of sperm morphometric subpopulations. J Miró, A Flotats, MM Rivera, M Ocaña, E Taberner, A Peña, T Rigau. Dept. Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain. Abstract OC4 Influence of seminal plasma added to highly diluted semen on sperm motility of young boar feed with selenium and vitamin E. F Centurión, S Echeverría, N Canul, R Ake, M Alfaro, R Santos, L Sarmiento. Faculty of Veterinary Medicine. Autonomous University of Yucatán, México. Abstract OC5 Filtration thought sephadex columns does not alter the subpopulation characteristics in fresh boar semen. L Ramió-Lluch1, M Rivera1, T Rigau1, S Balasch2, JE Rodríguez-Gil1. 1 Unit of Anim Reprod, Dept Anim Med & Surg, Fac of Vet Med, UAB, Bellaterra 2 Servicios genéticos porcinos, S.L. Roda de Ter, Barcelona, Spain. Abstract OC6 Scrotal heat stress in mice affects viability and DNA integrity of sperm, and sex ratio of the offspring. M Pérez-Crespo, E Pericuesta, R Rey, A Gutiérrez-Adán. Dpto de Reproducción Animal, INIA, Madrid, Spain. 13:30 Sesión de posters I (números impares) 14:00 Comida de trabajo SESIÓN PLENARIA II: INSEMINACIÓN ARTIFICIAL Moderador: Pedro García Herradón 16:30 Estrategias de mejora en inseminación artificial ovina. L Anel1, M Alvarez1, F Martinez-Pastor2, V Garcia-Macias1, E Anel1 and P de Paz3. 1 Animal Reproduction and Obstetrics, University of León, Leon; 2 Biology Reproduction, IREC, UCLM-CSIC-JCCM, Albacete; 3 Cellular Biology and Anatomy, University of León, León, Spain. 17:15 Nuevos retos en inseminación artificial porcina. J Roca, JM Vázquez, MA Gil, C Cuello, I Parrilla and EA Martínez Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, Campus de Espinardo, University of Murcia, Murcia, Spain. Sábado 21 de Octubre SESIÓN PLENARIA III: AVANCES EN EL CULTIVO IN VITRO DE EMBRIONES 9:30 Consecuencias para el desarrollo del dimorfismo sexual manifestado en el periodo embrionario preimplantacional. A Gutiérrez-Adán1, M Perez-Crespo1, R Fernandez-Gonzalez1, MA Ramirez1, P Moreira1, B Pintado1, P Lonergan2 and D Rizos1. 1 Dpto Reproducción Animal, INIA, Madrid, Spain; 2 School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland. 10:15 La vitamina A en el desarrollo embrionario temprano bovino. E Gómez, JN Caamaño, A Rodríguez, C De Frutos, N Facal and C Díez. Servicio Regional de Investigación y Desarrollo Agroalimentario, (SERIDA), Área de Genética y Reproducción, SERIDA, Asturias, Spain. SESIÓN PLENARIA IV: Financiación de la investigación Moderador: Emilio Espinosa Velázquez 11:00 Colaboración entre Centros públicos de Investigación y Empresas: Análisis de la situación y financiación de proyectos de investigación. Pedro Calleja y José Luís Calbet. Centro para el Desarrollo Tecnológico e Industrial (CDTI). Madrid. Spain. 12:00 Descanso/café 12:30 COMUNICACIONES ORALES II Moderador: Anselmo Gracia Molina Abstract OC7 Pregnancy-associated glycoprotein (PAG) cloning and expression in preimplantational ovine conceptuses. B Serrano1, J Yániz2, F López-Gatius3, JM Garbayo1. 1 CITA, Zaragoza 2 University of Zaragoza 3 University of Lleida, Spain. Abstract OC8 Effect of progesterone supplementation during early foetal period in Neospora caninum seropositive dairy cows. G Bech-Sàbat1, B Serrano2, I García-Ispierto3, P Santolaria4, J Yániz4, S Almería3, F López-Gatius1 1 University of Lleida, Spain; 2CITA, Zaragoza 3UAB, Barcelona 4 University of Zaragoza, Spain. Abstract OC9 Effect of melatonin implants in guirra ewes. M Rodriguez1, F Marco-Jiménez2, JS Vicente3, MP Viudes-de-Castro2. 1 Sistemas y Tecnologías de la Producción Animal, UPV. 2 Centro de Investigación y Tecnología Animal, IVIA. 3 Laboratorio de Biotecnología de la Reproducción, UPV, Spain. Abstract OC10 A negative energy balance but not suckling frequency (ad libitum, twice or once daily) modules ovarian resumption in Parda de Montaña cattle breed. A Sanz, J Alvarez, J Palacio, I Casasús, R Revilla. Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza, Spain. Abstract OC11 Changes in fibroblast growth factor-2 in ovine follicle development. RA Garcia1, MA Sanchez1, C Letelier2, P Garcia Palencia1, B Sanchez1, MP Vilar1, A Gonzalez-Bulnes2, JM Flores1. 1Facultad de Veterinaria. UCM 2Dpto de. Reproducción Animal. INIA, Madrid, Spain. Abstract OC12 Combination of IVF strategies to reduce porcine polyspermic fertilization: straw IVF system and short gamete coincubation time. C Almiñana, MA Gil, C Cuello, J Roca, JM Vazquez, EA Martinez. Dpt. of Medicine and Animal Surgery, University of Murcia, Spain. Abstract OC13 Induction of parturition in canary goats with luprostiol. M Batista, T Niño, D Alamo, F Cabrera, N Castro. Reproduction and Obstetrics, Veterinary Faculty of Las Palmas, Spain. 13:30 SESIÓN DE POSTERS II (números pares) 14:00 Comida de trabajo SESIÓN PLENARIA V: TECNOLOGÍAS REPRODUCTIVAS EN ANIMALES SILVESTRES Moderador: Agustín Josa Serrano 16:30 Cambios endocrinos estacionales y actividad reproductiva en rumiantes silvestres mediterráneos. J Santiago-Moreno1, A Gómez-Brunet1, A Toledano-Díaz1, R Picazo2, A Gonzalez-Bulnes1 and A López-Sebastián1 1 Department of Reproducción Animal, INIA; 2 Department of Fisiología Animal, Universidad Complutense, Madrid, Spain. 17:15 Consanguinidad y reproducción en ungulados en peligro de extinción: mantenimiento de variabilidad genética mediante la organización de bancos de recursos genéticos. ERS Roldan1, M Gomendio1, JJ Garde2, G Espeso3, S Ledda4, F Berlinguer4, A del Olmo1, AJ Soler2, L Arregui1, C Crespo1 and R González1. 1 Grupo de Ecología y Biología de la Reproducción, Museo Nacional de Ciencias Naturales (CSIC), Madrid; 2 Grupo de Biología de la Reproducción, Institututo de Investigación en Recursos Cinegéticos, (CSIC-UCLM-JCCM), Albacete; 3 Estación Experimental de Zonas Áridas (CSIC), Almeria, Spain; 4 Università degli Studi di Sassari, Sassari, Italy. 18:00 Aplicación de técnicas de reproducción asistida a poblaciones silvestres de ciervos. JJ Garde1, F Martínez-Pastor1, M Gomendio2, AF Malo2, AJ Soler1, MR Fernández-Santos1, MC Esteso1, AJ García1, L Anel3 and ERS Roldán2. 1 Reproductive Biology Group, Instituto de Investigación en Recursos Cinegéticos, IREC (UCLM-CSIC-JCCM), Campus Universitario, Albacete; 2 Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales (CSIC), Madrid; 3 Animal Reproduction and Obstetrics, University of León, León, Spain. 18:45 ASAMBLEA GENERAL DE AERA 21:00 CENA DE CLAUSURA POSTERS Sesión I (Viernes, 13:30 h.): Números impares. Sesión II (Sabado, 13:30 h.): Números pares. P1 Short term effect of melatonin implants on sexual activity in Mediterranean goat does without male effect. MC Gatica1, JL Guzmán1, R Farauste1, L Ijano1, S Martín2, A Martino2, LA Zarazaga1. 1 Departamento de Ciencias Agroforestales, Universidad de Huelva, Huelva, Spain. 2 Ceva Salud Animal Barcelon, Spain. P2 Short term effect of melatonin implants on sperm parameters in Mediterranean goat males. MC Gatica1, JL Guzmán1, S Martín2, A Martino2, P Vilar1 and LA Zarazaga1. 1 Departamento de Ciencias Agroforestales, Universidad de Huelva, Huelva, Spain. 2 Ceva Salud Animal, Barcelona Spain. P3 Increase in fertility and prolificacy in swine by hormone supplementation (Lechon-Plus) to extended semen on artificial insemination. MT Román1, JC Domínguez2, M Abad2, J Peláez2, B Alegre2, R González2. 1Área de Producción Animal, Escuela Técnica Sup Ing Agraria, Univ de Valladolid, Palencia. 2 Depto Reprod Obst, Univ de León, Spain. P4 Effect of selenium and vitamin E addition in the diet on young boar seminal characteristics. S Echeverría, F Centurión, R Ake, M Alfaro, R Santos, A Alzina, J Rodríguez. Faculty of Veterinary Medicine, University of Yucatán, México. P5 Effect of sperm quality selection on buck spermatozoa cryosurvival J Dorado, M Hidalgo, I Rodríguez. Animal Reproduction Group, University of Cordoba, Spain. P6 Inhibition of prolactin secretion during spring does not prevent the resurgence of horn growth in sub-adult and adult mouflon rams ( O. orientalis musimon). A Toledano-Díaz, J Santiago-Moreno, A Gomez-Brunet, A Lopez-Sebastian. Dpto. Reproducción Animal, INIA, Madrid, Spain. P7 Gestation early diagnosis in cattle by means of ecographyc exploration of the corpus luteum (CL). J Tejero, J C Domínguez, A F Álvarez, R Iglesias. Reproduction and Obstetrics, Faculty of Veterinary of León, Spain. P8 Defining bovine oocyte in vitro maturation conditions. S Ruibal, LA Quintela , JJ Becerra, AI Peña, PG Herradón. Unit of Reprod. & Obst., Veterinary Faculty, University of Santiago de Compostela, Lugo, Spain. P9 Freezability of Iberian boar semen: injuries on the acrosomal membrane appear early at 5ºC. I Casas1, S Sancho1, M Briz1, E Pinart1, N Garcia-Gil1, J Bassols1, A Pruneda1, E Bussalleu1, M Yeste1, F Saravia2, H Rodriguez-Martinez2, S Bonet1. 1 Biotechnology of Porcine Reproduction, University of Girona, Spain. 2 Division of Comparative Reproduction, Swedish University of Agricultural Sciences, Uppsala, Sweden. P10 Effect of hyaluronic acid added to boar cooled semen doses on sperm quality. M Yeste, M Briz, E Pinart, S Sancho, N Garcia-Gil, E Badia, J Bassols, A Pruneda, E Bussalleu, I Casas, S Bonet. Biotechnology of Porcine Reproduction, Department of Biology-INTEA, University of Girona, Spain. P11 Relationship between sex of the fetus and open days in dairy cattle. DM Arcovedo-Simmerman, G Arias-Sagarmínaga, ME Colula-Segundo, L González-León, R Monterrubio-Álvarez, C Rocha-Jaime, B Tapia González, A Córdova-Izquierdo. Departamento de Producción Agrícola Animal. Universidad Autónoma Metropolitana Unidad Xochimilco, México, D.F. P12 Effect of prostaglandin administration way on the oestrus synchronization in dairy heifers. J Ariza-Mendoza, D Berdeja-Reyes, W López-Martínez, S Velázquez-Rodríguez, A Córdova-Izquierdo. Departamento de Producción Agrícola y Animal. Universidad Autónoma Metropolitana Unidad Xochimilco, México. P13 Culture of bovine zygotes in the isolated mouse oviduct D Rizos1, B Pintado1, J de La Fuente1, P Lonergan2 , A Gutierrez-Adan1. 1 Dept de Animal Reproduction, INIA, Madrid, Spain. 2 School of Agric, Food Science and Vet Medicine, University College Dublin, Ireland. P14 Effect of chemical activation after intracytoplasmic sperm injection in pigs. E García-Roselló1, J Alfonso2, E García-Mengual3, I Salvador3, MA Silvestre3. 1Cardenal Herrera-CEU, Moncada. 2IMER, Valencia. 3CITA-IVIA, Segorbe, España. P15 Slow cooling to –4ºC improves quality of frozen buck ( Capra hircus) semen. F Cabrera, M Batista, F González, D Álamo, N Rodríguez, A Gracia. Unidad de Reproducción y Obstetricia, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Las Palmas. P16 Proliferation activity in testicular interstitial cells of Syrian hamster ( Mesocricetus auratus): changes during aging. E Beltrán1, C Ferrer1, A Zuasti1, CM Bernal-Mañas1, M Canteras2 , LM Pastor1. 1Department of Cell Biology, Institute of Aging, and 2Department of Statistics, School of Medicine, University of Murcia, Spain. P17 Aging changes in testicular interstitial cells of Syrian hamster: an ultrastructural study E Beltrán, C Ferrer, A Zuasti, R Horn, CM Bernal-Mañas, M Martín, LM Pastor. Department of Cell Biology, Institute of Aging, School of Medicine, University of Murcia, Spain. P18 Cell proliferation in the epithelium of the epididymis of Sus domesticus CM Bernal-Mañas1, A Zuasti1, C Ferrer1, E Beltrán1, E Pinart3, MD Briz3 S Bonet3, M Canteras2, LM Pastor1. 1 Dept of Cell Biology,2Dept of Statistics, School of Medicine, Univ of Murcia 3Biotechnology of Porcine Reprod, Univ of Girona, Spain. P19 Relation of apoptosis with seminiferous tubular degeneration in hamster aging ( Mesocricetus auratus). CM Bernal-Mañas1, E Morales1, C Ferrer1, A Zuasti1, E Beltrán1, M Canteras2, LM Pastor1. 1Departments of Cell Biology, Institute of Aging, and 2Department of Statistic, School of Medicine, University of Murcia, Spain. P20 Effects of sugar supplementation on the cryopreservation of Iberian red deer ( cervus elaphus hispanicus) epididymal spermatozoa. MR Fernández-Santos1, F. Martínez-Pastor1, V. García-Macías2, MC Esteso1, A Domínguez–Rebolledo1, A Soler1, P Paz 3, L Anel2, JJ Garde1. 1 Biología de la Reproducción. Instituto de Investigación en Recursos Cinegéticos (IREC), UCLM-CSIC-JCCM. Albacete, Spain. 2Reproducción Animal y Obstetricia, Universidad de León. León, Spain. 3 Biología Celular y Anatomía, Universidad de León, León, Spain. P21 Principal components analysed within the morphometric parameters of goat sperm head. M Hidalgo, J Dorado, I Rodríguez. Animal Reproduction Group, University of Córdoba, Spain. P22 Comparison of two procedures for sperm cryopreservation in the stallion. I Rodríguez, M Hidalgo, J Dorado, M Bottrel. Animal Reproduction Group, University of Córdoba, Spain. P23 Toxicity and osmotic stress of ethylene glycol on pig oocytes and parthenogenetic embryo development. J Alfonso 1,2, CC Duque 3, I Salvador 1, E García-Mengual 1, I Molina 3, MA Silvestre 1. 1CITA-IVIA Segorbe, 2 IMER Valencia, 3 H.U. La Fe, Valencia, Spain. P24 Breed-cross pregnancies increased plasmatic pregnancy-associated glycoprotein (PAG) concentration in pregnant Neospora-seropositive dairy cows. F López-Gatius1, JM Garbayo2, B Serrano2, P Santolaria3, J Yániz3, S Almería4, A Ayad6, NM Sousa6, JF Beckers6. 1Univ of Lleida, Spain; 2CITA, Zaragoza, 3Univ of Zaragoza,4Univ of Barcelona, Spain; 6 Fac of Veterinary Medicine, Liège, Belgium. P25 Apoptosis rate in prepubertal goat cumulus-oocyte complexes depending on oocyte diameter and cumulus-oocyte complex morphology. B Anguita, AR Jiménez-Macedo, R Romaguera, R Morató, MT Paramio, T Mogas, D Izquierdo. Facultat de Veterinària,UAB, Spain. P26 Effect of cryopreservation on the association of Syntaxin-2 to the lipid raft N Garcia-Gil 1, PS Tsai 1, A Boerke 1, S Bonet 2, BM Gadella 1. 1 Dept of Biochemistry & Cell Biology, Univ of Utrecht, The Netherlands 2 Biotechnology of Porcine Reproduction, Univ of Girona, Spain. P27 Measure of sperm concentration in boar ejaculates: traditional systems versus the new Nucleocounter®. F Tejerina1, R Gonzalez3, B Alegre3, M Castejón2, AJ Martín1, S Cardenas1, JC García3, S Bernal1, JC Domínguez1. 1 Fac. of Veterinary Medicine. University of León. 2 Dept of Electrical & Electronic Engineering, University of León. 3 CENTROTEC, SA. P28 Hydrometra associated with atresia of the uterus iin a ferret. D Allamo1, P Herráez2, M Santana1, F Rodríguez2, M Batista1, A Gracia1. 1Reproduction and Obstetrics, 2Department of Comparative Pathology, Veterinary Faculty of Las Palmas, Spain. P29 Segmentall apllasiia of the uterus associiated wiith hydrometra iin a goat. M Batista, D Alamo, N Rodríguez, T Niño, A Gracia. Reproduction and Obstetrics, Veterinary Faculty of Las Palmas, Spain. P30 Effect of the seminal additive ULTRA-RABBIT® on fertility and prolificacy rates of multiparous lactating does. R Gonzalez2 , F Tejerina1, B Alegre2, M Abad1, F Abad2, M Castejón3, S Bernal1, JC García1, JC Dominguez1. 1Reproduction & Obstetrics, Fac of Veterinary Medicine, Univ of Leon, 2CENTROTEC SA 3 Dept of Electr & Electronic Eng, Univ of Leon. P31 Apoptotic grade of embryos from prepubertal goat oocytes after ICSI and embryo biopsy. AR Jimenez-Macedo, D Izquierdo, B Anguita, R Romeguera, R Morato, T Mogas, MT Paramio. Departament de Ciència Animal i dels Aliments, Universitat Autonoma de Barcelona, Spain. P32 Sperm morphometry in endangered mammals. A del Olmo, M Gomendio, ERS Roldan. Grupo de Ecología y Biología de la Reproducción, Museo Nacional de Ciencias Naturales (CSIC), Madrid, Spain. P33 Cryopreservation of cat epididymal spermatozoa. Effect of diluent, cooling rate, glycerol addition and packaging method. N Gañán, R González, M Gomendio, ERS Roldan. Grupo de Ecología y Biología de la Reproducción, Museo Nacional de Ciencias Naturales (CSIC), Madrid, Spain. P34 Effect of glycerol on the viability and chromatin state of deep frozen boar semen BD Corcuera a, JF Pérez-Gutiérrez b. a Kubus, S.A., Spain; b Dpt. Medicina y Cirugía Animal, Facultad Veterinaria, UCM, Spain. P35 Sperm head morphometry parameters in two selected rabbit lines. R Lavara1, F Marco-Jiménez2, C Cortell 2, HM Safaa3, JS Vicente1. 1Dpto de Ciencia Animal, Universidad Politécnica Valencia and 2CITA, IVIA, Spain 3 Animal Prod. Dpt, Cairo University, Egypt. P36 Expression of GM-CSF receptor in ovine spermatozoa. JE Rodríguez-Gil1 , G Silvers2,3, E Flores1, MJ Palomo1, A Ramírez2, M Castro2, M Brito2, J Correa3 , II Concha2. 1 Unitat Reproducció Animal, Univ Autònoma de Barcelona, Bellaterra, Spain; 2 Inst de Bioquímica, Fac de Ciencias y 3 Inst de Reproducción Animal Facultad de Ciencias Veterinarias, Univ Austral de Chile, Valdivia, Chile. P37 GM-CSF effect on semen quality parameters of ovine spermatozoa after freezing/thawing process. E Flores1, MJ Palomo1, JE Rodríguez-Gil1 , G Silvers2,3, A Ramírez2, M Castro2, M Brito2, J Correa3 , II Concha2. 1 Unitat Reproducció Animal, Univ Autònoma de Barcelona, Bellaterra, Spain; 2 Inst de Bioquímica, Fac de Ciencias y 3 Inst de Reproducción Animal Facultad de Ciencias Veterinarias, Univ Austral de Chile, Valdivia, Chile. P38 Effect of the lactose addition on the viability and chromatin state of deep frozen boar semen. BD Corcuera a, JF Pérez-Gutiérrez b. a Kubus, S.A., Spain, b Dpto. Medicina y Cirugía Animal, Facultad Veterinaria, UCM, Spain. P39 Effects of the addition of ethylene glycol for cryopreservation of canine sperm. A Martins-Bessa1, A Rocha2, JF Pérez3, A Mayenco-Aguirre3. 1UTAD, Vila Real, Portugal; 2 University of Oporto, Portugal; 3UCMadrid, Spain. P40 Relationship between mitochondrial status and classic parameters of dog sperm evaluation. AM Mayenco-Aguirre1, AC Martins-Bessa2, JF Pérez1. Veterinary Faculty 1 UCM, Spain, 2UTAD, VilaReal, Portugal. P41 Genetic characterisation of brown trout in Aragon (Northeast Spain). O Mitjana, C Monclus, JM Blasco, A Josa, E Espinosa. Unidad de Reproducción, Facultad Veterinaria, and CITA-DGA, Zaragoza, España. P42 Effect of refrigeration on epididymal stallion spermatozoa motile subpopulations. E Taberner, I García, M López-Béjar, M Rivera, M Ocaña, C López, J Miró. Veterinary Faculty, Autonomous University of Barcelona, Bellaterra, Spain. P43 Dynamics of corpus luteum in sheep with the oestrus synchronized either with progestagens or prostaglandin analogues. C Letelier1, A Gonzalez-Bulnes1, I Contreras1, RA Garcia2, P Garcia-Palencia2, B Sanchez1, MA Sanchez1, JM Flores1. 1 Reproducción Animal, INIA 2 Facultad de Veterinaria, UCM, Spain. P44 Spindle configuration after OPS vitrification of calf and cow oocytes using TaxolTM, a cytoskeletal stabilizer. R Morató, D Izquierdo, MJ Palomo, JL Albarracín, AR Jiménez-Macedo, B Anguita, MT Paramio, T Mogas. Departament de Medicina i Cirurgia Animals, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, UAB, Spain. P45 Effect of growth factors on bovine embryos cultured in vitro in B2 and SOFm media. M Barrio, AI Peña, JJ Becerra, LA Quintela, C Rey, J Deiros, C Díaz, S Ruibal, PG Herradón. Unit of Reproduction and Obstetrics. University of Santiago de Compostela. Spain. P46 Fractionated glycerol addition at different temperatures does not affect post thawing results in dog semen. V García-Macías1, MV Arroyo1, M Álvarez1, F Martínez-Pastor2, N González1, M Nicolás1, L Anel1. 1Animal Reproduction, University of León; 2IREC (CSIC-UCLM-JCCM), Albacete, Spain. P47 Effect of two anesthesic protocols in blood cortisol levels of electroejaculated bulls. M Alvarez1, V García-Macías1, JC Boixo2, F Martínez-Pastor1,3, J Muro1, J Bernardo1, S Alves1, E Anel1, L Anel1. 1 Reproduction and Obstetrics, University of León, Spain; 2CENSYRA, León, Spain; 3IREC (CSIC-UCLM-JCCM), Albacete, Spain. P48 Role of equine growth hormone on equine oocyte maturation: receptor localization by immunocytochemistry and real time PCR. PL Lorenzo1, GR Pereira2, S Bilodeau-Goeseels3, GF Carneiro2, JP Kastelic3, IKM Liu2. 1Depto Fisiología Animal, Facultad de Veterinaria-UCM, Madrid, Spain; 2 Population Health & Reprod, School of Veterinary Medicine, University of California, Davis, USA; 3Agriculture & Agri-Food Research Centre, Lethbridge, Canada. P49 Choice of housekeeping genes for their low variability in experiments with embryos and retinoids. A Rodríguez, LJ Royo, C Díez, JN Caamaño, F Goyache, N Facal, C de Frutos, E Gómez. Genética y Reproducción SERIDA. Asturias. SPAIN. P50 Sex ratio modification in rabbit litters obtained by artificial insemination: preliminary results. LA Quintela, AI Peña, MD Vega, JJ Becerra, S Ruibal, C Rey , C Díaz, J Deiros, M Barrio, PG Herradón. Reproduction and Obstetrics, Veterinary Faculty, Lugo, Spain. P51 Changes in head morphometry are related to post-thawing quality of cryopreserved epididymal spermatozoa from Iberian red deer. MC Esteso, MR Fernández-Santos, F Martínez-Pastor, A Domínguez-Rebolledo, AJ Soler, JJ Garde. Instituto de Investigación en Recursos Cinegéticos (IREC), UCLM-CSICJCCM, Albacete, Spain. P52 Reproductive lesions in dogs with metastatic malignant mammary tumours. M Clemente, MA Jiménez, PJ de Andrés, MD Pérez-Alenza, JC Illera, L Peña. Veterinary Faculty, UCM, Spain. P53 Development of bovine embryos produced in vitro: effect of growth factors during culture. J Álvarez1, S de la Varga1, A Katchicualula1, L Fernández1, F de la Fuente1, C Díez2, CO Hidalgo2, C Tamargo2, M Carbajo1. 1 Veterinary Faculty, León; 2 SERIDA, Gijón, Spain. P54 Variations of sex reproductive hormones during the year in a wild population of European pine marten ( Ma rtes martes). G Silvan1, I Barja2, S Rosellini3, A Piñeiro3, MJ Illera1, L Camacho1, JC Illera1. 1 Fisiología Animal, UCM, Madrid 2 Biología y Ciencias del Medio Ambiente, Univ SEK, Segovia,3Biodiv y Biología Evolutiva, CSIC, Spain. P55 Estimation of breed and seasonal effect on semen quality in Asturiana de los Valles and Holstein bulls. C Tamargo1, L Prieto1, M Carbajo2, C Díez1, S de la Varga2, F de la Fuente2, J Álvarez2, CO Hidalgo1. 1SERIDA, Gijón, 2 Veterinary Faculty, León, Spain. P56 Effect of Cholesterol and Equex addition in equine frozen/ thawed sperm membranes protection. I Galindo, L Gil, N Gonzalez, D Mualuzanga. Faculty of Veterinary, University of Zaragoza, Spain. P57 Influence of the age and the body condition on the artificial insemination reproductive results of Rasa Aragonesa ewes. MA Ciudad 1, L Gil 1, E Espinosa1, J.L Alabart 2, J Folch 2. 1 Universidad de Zaragoza, Facultad Veterinaria. 2 CITA, Gobierno de Aragón, Zaragoza, Spain. P58 Influence of the intervals synchronization-artificial insemination (AI) and ejaculate collection-AI on reproductive results of Rasa Aragonesa ewes. MA Ciudad 1, L Gil 1, E Espinosa1, JL Alabart 2, J Folch 2. 1 Universidad de Zaragoza, Facultad Veterinaria. 2 CITA, Gobierno de Aragón, Zaragoza, Spain. P59 Effects of the micotoxin zearalenone on swine genital tract. MV Falceto1, JA Bascuas1, J Grandia2, R Menjon2. 1 Facultad de Veterinaria, Universidad de Zaragoza, 2 Cooperativa Agraria San Miguel, Tauste, Zaragoza, Spain. P60 Effects of breed on follicular dynamics during the follicular phase of oestrous cycle in sheep. P Gonzalez-Añover1, T Encinas1, A Veiga-Lopez2, I Ammoun1, I Contreras2, JM Ros1, C Ariznavarreta3, JAF Tresguerres3, A Gonzalez-Bulnes1,2. 1Facultad de Veterinaria, UCM,2Dpto. de Reproduccion Animal, INIA, 3 Facultad de Medicina, UCM, Spain. P61 Effects of breed on kinetics of ovine FSH and ovarian response in superovulated sheep. I Ammoun1, T Encinas1, A Veiga-Lopez2, JM Ros1, I Contreras2, P Gonzalez-Añover1, MJ Cocero2, AS McNeilly3 A Gonzalez-Bulnes1,2. 1 Facultad de Veterinaria. UCM and 2 Departamento de Reproduccion Animal, INIA, Spain; 3 Centre for Reproductive Biology, UK. P62 Systemic and intraovarian effects of the corpus luteum on follicular populations during the oestrous cycle of West African ewes. I Contreras1, T Díaz1, G López2, A Caigua1, A Gonzalez-Bulnes3. 1UCV, Venezuela, 2INIA-Lara, Venezuela, 3Dpto de Reproduccion Animal, INIA, Spain. P63 Predictive value of ejaculate parameters on sperm cryopreservation outcome in pigs. M Hernández, J Roca, JM Vazquez, EA Martinez. Department of Medicine and Animal Surgery. Faculty of Veterinary Medicine, University of Murcia, Spain. P64 In vitro pig embryo production using the straw IVF system and a short gamete co-incubation time. C Almiñana, MA Gil, C Cuello, J Roca, JM Vazquez, EA Martinez. Dpt. of Medicine and Animal Surgery, University of Murcia, Spain. P65 Metastatic granulosa cell tumour: an unusual case report in a bitch. X Lucas, A Agut, F de Membiela, J Carrillo, E Belda, M Hernandez. Dpt. of Animal Medicine and Surgery, University of Murcia, Spain. P66 Effects of two vaccines against blue tongue on sperm viability for Manchega ram. A Maroto-Morales, O García-Álvarez, R Arias, AJ Soler, MD Perez-Guzman. CERSYRA (JCCM), Spain. P67 The effect of melatonin administration on growth and reproductive performance in rainbow trout (Oncorhynchus mykiss). E Espinosa, A Josa, L Gil, O Mitjana. Reproducción Ictiológica, Universidad de Zaragoza, Zaragoza, Spain. P68 The effect of long-term storage on the in vitro development of SOPSvitrified porcine blastocysts. C Cuello, MA Gil, C Almiñana, J Sanchez-Osorio, I Parrilla, J Roca, JM Vazquez, EA Martinez. Dept. of Animal Medicine & Surgery. University of Murcia, Spain. P69 Double freezing of sex sorted bovine semen. I Parrilla1,2, J Roca2, EA Martinez2, JM Vazquez2 WMC Maxwell3, D Rath1. 1Inst Anim Breed Mariensee (FAL) Germany 2Dpt Anim Med & Surg, Univ of Murcia, Spain. 3 Fac Vet Science, Univ of Sydney, Australia. P70 Fertility in 36 week-old male broiler breeders: I. Body and testis weight. M Pizarro1, J Sarabia1, JC Abad2, M González1, E Tabanera1. 1 Dpto. Medicina y Cirugía Animal, Fac. Veterinaria, UCM, Madrid, 2 OBB Española S.A., Alcalá de Henares, Madrid, Spain. P71 Fertility in 36 Week-old male broiler breeders: II. Histological study of the testis. M Pizarro1, J Sarabia1, JC Abad2, A Rodríguez1, M Castaño1. 1 Dpto. Medicina y Cirugía Animal, Fac. Veterinaria, UCM, Madrid, 2 COBB Española S.A., Alcalá de Henares, Madrid, Spain. P72 Testosterone, dehydroepiandrosterone (DHEA) and 17-β-estradiol blood serum concentrations in relation to disease in the horse. I Ayala1, N Martos1, C Gutierrez-Panizo1, JG Clavel2, JC Illera3. 1Dpto. Medicina y Cirugía Animal, Universidad de Murcia; 2 Dpto. A. Cuantitativo and 3Dpto. Fisiología Animal, UCM, Madrid, Spain. P73 Comparison between the sperm zona pellucida binding assay and homologous in vitro penetration test for evaluation of sperm quality in the ovine. J Yániz1, JI Martí2, MA Silvestre1, J Folch2, P Santolaria1, JL Alabart2, F López-Gatius4. 1 Dpt Anim Prod, Univ of Zaragoza, Huesca 2 Center for Agric Res, Dpt Anim Prod, Zaragoza 3 Dpt Anim Prod, Univ of Lleida, Lleida, Spain. P74 Effects of insertion of deep intrauterine insemination device into one uterine horn on the reproductive performance of multiparous sows. L Rodriguez-Vilar, C López-Sánchez, A Bolarín, M Hernández, JM Vazquez, EA Martinez, J Roca. Department of Medicine and Animal Surgery, University of Murcia, Spain. P75 Identification of proteins that protect ram spermatozoa against cold-shock by two-dimensional electrophoresis. JA Cardozo, M Fernández-Juan, JA Cebrián-Pérez, T Muiño-Blanco. Department of Biochemistry and Molecular and Cell Biology, School of Veterinary Medicine, University of Zaragoza, Spain. P76 Caspase activity and phosphatidylserine exposure in ram spermatozoa during cold-shock, in vitro capacitation and acrosome-reaction. E Martí, R Pérez-Pé, T Muiño-Blanco, JA Cebrián-Pérez. Department of Biochemistry and Molecular and Cell Biology, School of Veterinary Medicine, University of Zaragoza, Spain. |
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